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Product Name
GGA2 Polyclonal Antibody
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Description
Polyclonal antibody to GGA2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
GGA2 antibody; VEAR antibody; ADP-ribosylation factor-binding protein GGA2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GGA2 (NP_055859.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - GGA2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using GGA2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry - GGA2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - GGA2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human esophageal cancer using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry - GGA2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using GGA2 Antibody at dilution of 1:200 (40x lens).
Immunofluorescence - GGA2 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using GGA2 antibody . Blue: DAPI for nuclear staining.
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Background
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Regulates retrograde transport of phosphorylated form of BACE1 from endosomes to the trans-Golgi network.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"