Product Name

GFPT1 antibody

Documents

Description

GFPT1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue, human hepat℃irrhosis tissue. Positive WB detected in human skeletal muscle tissue, HepG2 cells. Observed molecular weight by Western-blot: 77kd

Tested applications

ELISA, IHC, IF, WB

Species reactivity

Human,Mouse,Rat,Zebrafish; other species not tested.

Alternative names

GFA antibody; GFAT antibody; GFAT 1 antibody; GFAT1 antibody; GFAT1m antibody; GFPT antibody; GFPT1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of GFPT1 recombinant protein (Accession Number: NM_002056). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:110951(GFPT1 antibody) at dilution of 1:500

Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110951(GFPT1 antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human gliomas using Catalog No:110951(GFPT1 antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody Catalog No:110951 at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Background

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

References

Senderek J, Müller JS, Dusl M. Hexosamine biosynthetic pathway mutations cause neuromuscular transmission defect. American journal of human genetics. 88(2):162-72. 2011.
Ganguly R, Sahu S, Chavez RJ, Raman P. Trivalent chromium inhibits TSP-1 expression, proliferation, and O-GlcNAc signaling in vascular smooth muscle cells in response to high glucose in vitro. American journal of physiology. Cell physiology. 308(2):C111-22. 2015.
Dusl M, Senderek J, Müller JS. A 3'-UTR mutation creates a microRNA target site in the GFPT1 gene of patients with congenital myasthenic syndrome. Human molecular genetics. 24(12):3418-26. 2015.

GFPT1 antibody

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