GFOGER peptide

GFOGER peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GFOGER

GFOGER, O is Hyp

6

95.27%

677.71

Synthetic

Powder

GFOGER is a specific amino acid sequence within collagens. It is recognized by the integrin α₂β₁ and α₁β₁, and is crucial for cell adhesion mediated by these integrins. The recognition of GFOGER is highly specific, with the glutamyl residue being essential. Its triple-helical conformation is also necessary for recognition. GFOGER acts as a potent inhibitor of cell adhesion to collagens, particularly for α₂β₁-mediated adhesion to collagens I and IV and α₁β₁-mediated adhesion to collagen I. However, it has little effect on α₁β₁-mediated adhesion to collagen IV. Overall, GFOGER plays a significant role in the interaction between collagens and cells via integrins. Hydrogels presenting GFOGER prolong hMSC survival and engraftment in a segmental bone defect and result in improved bone repair compared to other peptides.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Knight CG, Morton LF, Peachey AR, Tuckwell DS, Farndale RW, Barnes MJ. The collagen-binding A-domains of integrins alpha(1)beta(1) and alpha(2)beta(1) recognize the same specific amino acid sequence, GFOGER, in native (triple-helical) collagens. J Biol Chem. 2000 Jan 7;275(1):35-40. doi: 10.1074/jbc.275.1.35. PMID: 10617582.
• Clark, A.Y., Martin, K.E., García, J.R. et al. Integrin-specific hydrogels modulate transplanted human bone marrow-derived mesenchymal stem cell survival, engraftment, and reparative activities. Nat Commun 11, 114 (2020). https://doi.org/10.1038/s41467-019-14000-9

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.