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Product Name
GAR1 antibody
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Description
GAR1 Rabbit Polyclonal antibody. Positive IP detected in A375 cells. Positive WB detected in A375 cells, human skin tissue. Positive IHC detected in human skin cancer tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 28kd
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
GAR1 antibody; NOLA1 antibody; snoRNP protein GAR1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of GAR1 recombinant protein (Accession Number: NM_018983). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:110866(GAR1 antibody) at dilution of 1:300
IP Result of anti-GAR1 (IP:Catalog No:110866, 3ug; Detection:Catalog No:110866 1:300) with A375 cells lysate 6000ug.
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110866(GAR1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:110866(GAR1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:110866(GAR1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.
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References
- Kim MS, Kim SS, Yoo NJ, Lee SH. Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers. Pathology. 44(6):576-7. 2012.
- Jobert L, Skjeldam HK, Dalhus B. The human base excision repair enzyme SMUG1 directly interacts with DKC1 and contributes to RNA quality control. Molecular cell. 49(2):339-45. 2013.
- Lin P, Mobasher ME, Hakakian Y. Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage. Histochemistry and cell biology. 144(6):543-58. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"