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Product Name
Recombinant FUT8 protein (His tag)
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Description
Recombinant Alpha-(1,6)-fucosyltransferase (FUT-8) fragment (residues 104–559) from Caenorhabditis elegans, encompassing the GT23 and SH3 functional domains. This high-purity protein is ideal for studying core fucosylation in N-glycans and enzyme-substrate interactions in vitro.
Alpha-(1,6)-fucosyltransferase (FUT-8) catalyzes the addition of fucose in α1-6 linkage to the innermost GlcNAc residue of N-glycans, a key step in core fucosylation that is blocked if the residue is pre-fucosylated. This modification influences glycoprotein function and cellular susceptibility, as demonstrated by its role in binding the nematotoxic galectin Cgl2. The enzyme localizes to the Golgi apparatus as a type II transmembrane protein, with its catalytic domain oriented in the Golgi lumen. The recombinant fragment (104–559) retains critical domains for structural and functional studies, enabling research into glycan biosynthesis and fucosylation-related pathways.
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Protein name
Alpha-
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Uniprot ID
G5EFE7
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Gene Name
fut-8
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 104-559 aa (G5EFE7) were fused with 6His tag.
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Protein Species
Caenorhabditis elegans
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Molecular weight
Predictes a molecular mass of 53.44 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 54 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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