Rat Frizzled-4/FZD4/CD344 (Fc Tag) recombinant protein

Rat Frizzled-4/FZD4/CD344 (Fc Tag) recombinant protein

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Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues (By similarity).

Frizzled-4

FZE4; GPCR; FZD4; FZD4S; FRIZZLED 4; FRIZZLED FAMILY RECEPTOR 4; FZ4; CD344; HFZ4; FRIZZLED HOMOLOG 4 (DROSOPHILA); MFZ4; SEVEN TRANSMEMBRANE SPANNING RECEPTOR; FRIZZLED HOMOLOG 4; EVR1; WNT RECEPTOR FRIZZLED-4; FZ-4; FRIZZLED-4; FEVR

Q9QZH0

Fzd4

Human Cells

A DNA sequence encoding the rat FZD4 (Q9QZH0) (Met1-Glu181) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat FZD4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 385 amino acids and has a predicted molecular mass of 43.2 kDa. The apparent molecular mass of the protein is approximately 113, 58 and 36 kDa in SDS-PAGE under reducing conditions.

(13.3+78.9+5.2) % as determined by SDS-PAGE