Recombinant FOXO protein (His tag)

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Recombinant Forkhead box protein O (dFOXO) fragment (residues 93-178) from Drosophila melanogaster, a key transcription factor in insulin signaling and lifespan regulation. This 86-amino acid fragment is engineered for high stability and solubility, ideal for structural studies and biochemical assays investigating metabolic and aging pathways.

Forkhead box protein O (dFOXO), also known as Protein FKHR, is a crucial transcription factor in Drosophila melanogaster that regulates the insulin signaling pathway. It activates downstream targets including Thor/d4EBP and the insulin receptor (InR), playing essential roles in cell cycle control, growth modulation, and stress response. Under nutrient deprivation or oxidative stress, dFOXO becomes activated and inhibits growth through target genes. Notably, dFOXO activation in the adult fat body regulates lifespan and integrates insulin responses to maintain glucose and lipid homeostasis by controlling homologs of human acid lipases. The protein localizes to both the cytoplasm and nucleus, with phosphorylation status determining subcellular localization—phosphorylation triggers cytoplasmic translocation while dephosphorylation promotes nuclear import, enabling precise regulation of its transcriptional activity.

93-178