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Product Name
FLCN Polyclonal Antibody
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Description
Polyclonal antibody to FLCN
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Tested applications
WB, IHC
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Species reactivity
Human
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Alternative names
FLCN antibody; BHD antibody; FLCL antibody; folliculin antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human FLCN
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, pH7.3.
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Storage instructions
Store at 4℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - FLCN Polyclonal Antibody
Western blot analysis of extracts of HeLa cells, using FLCN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.
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Background
May be a tumor suppressor. May be involved in energy and/or nutrient sensing through the AMPK and mTOR signaling pathways. May regulate phosphorylation of RPS6KB1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"