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Product Name
Fibulin 5 antibody
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Description
Fibulin 5 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue, human heart tissue, human pancreas tissue. Positive IP detected in mouse heart tissue. Positive WB detected in mouse heart tissue, mouse placenta tissue, rat brain tissue. Observed molecular weight by Western-blot: 64-66 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARMD3 antibody; DANCE antibody; EVEC antibody; FBLN5 antibody; FIBL 5 antibody; fibulin 5 antibody; FLJ90059 antibody; UP50 antibody; Urine p50 protein antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Fibulin 5 recombinant protein (Accession Number: NM_006329). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:110661(FBLN5 antibody) at dilution of 1:400
IP Result of anti-FBLN5 (IP:Catalog No:110661, 3ug; Detection:Catalog No:110661 1:300) with mouse heart tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110661(FBLN5 antibody) at dilution of 1:50 (under 10x lens)
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Background
Fibulin-5, also known as EVEC and DANCE, is a extracellular matrix glycoprotein, secreted by various cell types such as vascular smooth muscle cells, fibroblasts and endothelial cells. Structurally, Fibulin-5 contains an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies. This antibody got 50kd or 66kd band in western blotting, the 66kd corresponding to fully glycosylated protein.
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References
- Chen JY, Tsai PJ, Tai HC. Increased aortic stiffness and attenuated lysyl oxidase activity in obesity. Arteriosclerosis, thrombosis, and vascular biology. 33(4):839-46. 2013.
- Han W, Guo C, Liu Q. Aberrant elastin remodeling in the lungs of O₂-exposed newborn mice; primarily results from perturbed interaction between integrins and elastin. Cell and tissue research. 359(2):589-603. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"