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Product Name
FECH antibody
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Description
FECH Rabbit Polyclonal antibody. Positive WB detected in human heart tissue, HeLa cells, human liver tissue, K-562 cells. Observed molecular weight by Western-blot: 46kd
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Tested applications
ELISA, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
EPP antibody; FCE antibody; FECH antibody; FerrOChelatase antibody; mitOChondrial antibody; Heme synthase antibody; Protoheme ferro lyase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FECH recombinant protein (Accession Number: NM_000140). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:110613(FECH antibody) at dilution of 1:500
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Background
FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.
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References
- Khechaduri A, Bayeva M, Chang HC, Ardehali H. Heme levels are increased in human failing hearts. Journal of the American College of Cardiology. 61(18):1884-93. 2013.
- Qiao A, Khechaduri A, Kannan Mutharasan R, Wu R, Nagpal V, Ardehali H. MicroRNA-210 decreases heme levels by targeting ferrochelatase in cardiomyocytes. Journal of the American Heart Association. 2(2):e000121. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"