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Product Name
Mouse CD16-2/FCGR4 (His & AVI Tag) recombinant protein
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Protein name
Fc of IgG, low affinity IIIa, receptor
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Protein short names
FCRL3; 4833442P21RIK
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Uniprot ID
A0A0B4J1G0
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Gene Name
Fcgr4; Fcgr3a; mCG_8631
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 35-40 kDa due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
1. Measured by its ability to bind human IgG1 (Fc) (Cat:500583) in a functional ELISA.
2. Labeling ratio of biotin to protein: 0.6 -
Validations
Mouse CD16-2 / FCGR4 Protein (His & AVI Tag), Biotinylated SDS-PAGE
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