Recombinant FANCM protein (His tag)

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Recombinant Human Fanconi anemia group M protein (FANCM) fragment (residues 1818-2048) is a highly stable and soluble 231-amino acid segment ideal for structural and biochemical studies. This fragment, derived from the ATP-dependent RNA helicase FANCM, supports research into DNA repair mechanisms and Fanconi anemia pathways, offering a reliable tool for investigating protein interactions and enzymatic activities.

The full-length FANCM protein is a DNA-dependent ATPase and key component of the Fanconi anemia (FA) core complex, essential for activating the FA pathway in response to DNA damage. It facilitates the monoubiquitination of the FANCI-FANCD2 complex, enhancing cellular resistance to DNA cross-linking agents and preventing chromosomal breakage. FANCM exhibits ATP-dependent DNA branch migration activity, processing branched DNA structures like replication forks, particularly when complexed with CENPS and CENPX. It also binds various DNA substrates, including single-strand DNA and splayed-arm DNA, in association with FAAP24. Localized to the nucleus, FANCM plays a critical role in genome maintenance and DNA repair, with implications for understanding Fanconi anemia and cancer biology.