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Product Name
ETFB antibody
- Documents
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Description
ETFB Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in mouse skeletal muscle tissue, human heart tissue, mouse brain tissue, rat brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Beta ETF antibody; ETFB antibody; FP585 antibody; MADD antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ETFB recombinant protein (Accession Number: NM_001985). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
Immunofluorescent analysis of HepG2 cells, using ETFB antibody Catalog No:110484 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:110484(ETFB Antibody) at dilution of 1:1000
IP Result of anti-ETFB (IP:Catalog No:110484, 3ug; Detection:Catalog No:110484 1:1000) with mouse brain tissue lysate 4000ug.
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Background
Electron transfer flavoprotein (ETF) exists in the mitochondrial matrix as a heterodimer of 30 kDa alpha subunits (ETFA)and 28 kDa beta subunits (ETFB) and contains 1 flavin adenine dinucleotide (FAD) and 1 adenosine 5-prime monophosphate (AMP) per heterodimer.It serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase.ETFB has 2 isoforms produced by alternative splicing. Defects in ETFB are the cause of glutaric aciduria type 2B (GA2B).
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References
- García-Gómez S, Reyes A, Martínez-Jiménez MI. PrimPol, an archaic primase/polymerase operating in human cells. Molecular cell. 52(4):541-53. 2013.
- Rhein VF, Carroll J, He J, Ding S, Fearnley IM, Walker JE. Human METTL20 methylates lysine residues adjacent to the recognition loop of the electron transfer flavoprotein in mitochondria. The Journal of biological chemistry. 289(35):24640-51. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"