Recombinant ERBB2 protein (His tag)

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Recombinant Human ERBB2 Kinase Domain (residues 713-1019) is a high-purity protein fragment encompassing the complete tyrosine kinase domain of the Receptor tyrosine-protein kinase erbB-2 (HER2). This fragment serves as an essential tool for investigating kinase activity, signal transduction mechanisms, and therapeutic targeting in cancer research and drug discovery.

The full-length ERBB2/HER2 protein is a crucial cell surface receptor tyrosine kinase (EC 2.7.10.1) that functions as part of multiple receptor complexes, requiring coreceptors for ligand binding. It plays vital roles in cellular signaling pathways, regulating peripheral microtubule outgrowth and stabilization through the MEMO1-RHOA-DIAPH1 pathway, which influences GSK3B phosphorylation and subsequent microtubule capture. Beyond membrane signaling, ERBB2 translocates to the nucleus where it participates in transcriptional regulation, including activation of PTGS2/COX-2 promoter and rRNA gene transcription, thereby enhancing protein synthesis and cell growth. The protein primarily localizes to the cell membrane as a single-pass type I membrane protein, with isoforms showing additional localization in early endosomes, perinuclear regions, and the nucleus. Its involvement in multiple cellular compartments underscores its significance in both signal transduction and gene expression regulation, making it a critical target in oncology research.