EPO mimetic peptide-1
Not For Human Use, Lab Use Only.
Cat.#: 319826
Special Price 183.50 USD
Availability:
1-2 weeks
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Product Name
EPO mimetic peptide-1
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-GGTYSCHFGPLTWVCKPQGG-OH
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Sequence
H-Gly-Gly-Thr-Tyr-Ser-Cys-His-Phe-Gly-Pro-Leu-Thr-Trp-Val-Cys-Lys-Pro-Gln-Gly-Gly-OH
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Length (aa)
20
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Peptide Purity (HPLC)
95.28%
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Molecular Formula
C94H135N25O26S2
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Molecular Weight
2095.35
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CAS No.
186526-28-3
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Source
Synthetic
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Form
Powder
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Description
EPO mimetic peptide-1 (EMP1) is a peptide with the consensus sequence YXCXXGPXTWXCXP, where X represents variable amino acids. This peptide was identified through phage display libraries and binds to the erythropoietin receptor (EPOR), activating signaling pathways identical to those induced by the natural ligand, erythropoietin (EPO). Despite lacking sequence homology with EPO, EMP1 functions as a full agonist in vitro, promoting cell proliferation in EPOR-expressing cell lines. The peptide's activity depends on its disulfide-bonded cyclic structure, which stabilizes a β-turn motif critical for receptor interaction.
Structural studies reveal that EMP1 binds the EPOR extracellular domain, inducing receptor dimerization through symmetric interactions. The peptide's conserved GPXTW motif and a tyrosine residue outside the disulfide loop are essential for receptor binding and activation. EMP1 exhibits stability in serum and demonstrates biological activity in vivo, stimulating erythropoiesis in murine models. These properties highlight its potential as a structural template for designing small-molecule EPOR agonists.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides
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References
- Wrighton NC, Farrell FX, Chang R, Kashyap AK, Barbone FP, Mulcahy LS, Johnson DL, Barrett RW, Jolliffe LK, Dower WJ. Small peptides as potent mimetics of the protein hormone erythropoietin. Science. 1996 Jul 26;273(5274):458-64. doi: 10.1126/science.273.5274.458. PMID: 8662529.
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About TFA salt
Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.
Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.
Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.
In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"