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Product Name
Danio rerio (zebrafish) EFNB2A / Ephrin B2a (Fc Tag) recombinant protein
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Description
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration (By similarity).
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Protein name
Ephrin-B2a
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Protein short names
ID:IBD5072; EFNB2; CH211-149B20.1
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Uniprot ID
O73874
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Gene Name
efnb2a; efnb2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with the Fc region of human IgG1 at the C-terminus.
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Protein Species
Danio rerio (zebrafish)
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Molecular weight
The recombinant zebrafish EFNB2A consists of 439 amino acids and has a calculated molecular mass of 48.9 kDa. It migrates as an 97 and 58 KDa band in SDS-PAGE under reducing conditions.
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Purity
(16.2+75.4) % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized human EphB4-His (Cat:502026) at 10 μg/ml (100 μl/well) can bind zebrafish EFNB2A-Fc, The EC50 of zebrafish EFNB2A-Fc is 12.8-30.1 ng/ml. -
Validations
Danio rerio (zebrafish) EFNB2A / Ephrin B2a Protein (Fc Tag) SDS-PAGE
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