Recombinant ENOPH protein (His tag)

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Recombinant Enolase-phosphatase E1 (EC 3.1.3.77) fragment from Drosophila melanogaster, encompassing residues 1-256. This bifunctional enzyme fragment is ideal for investigating methionine salvage pathway dynamics, offering a stable and soluble unit optimized for structural and biochemical studies.

Enolase-phosphatase E1 is a bifunctional enzyme that catalyzes two sequential steps in the methionine salvage pathway: the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), followed by dephosphorylation to produce the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). This enzymatic activity is crucial for recycling methionine precursors in cellular metabolism. The full-length protein localizes to both the cytoplasm and nucleus, suggesting potential roles in multiple cellular compartments. The recombinant fragment (residues 1-256) represents a computationally selected stable domain that preserves key functional regions for experimental applications.

1-256