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Product Name
EIF4G3 Polyclonal Antibody
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Description
Polyclonal antibody to EIF4G3
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
EIF4G3 antibody; eIF-4G 3 antibody; eIF4G 3 antibody; eIF4GII antibody; eIF-4G3 antibody; eIF4G3 antibody; eukaryotic translation initiation factor 4 gamma 3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1000-1200 of human EIF4G3 (NP_003751.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - EIF4G3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using EIF4G3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
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Background
Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"