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Product Name
Recombinant EED protein (His tag)
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Description
Recombinant Human Polycomb protein EED (hEED) fragment (residues 78-441) is a high-quality, stable protein designed for structural and biochemical studies. This 364-amino acid fragment retains key functional properties, offering researchers a reliable tool to investigate epigenetic regulation mechanisms.
The full-length EED protein is a crucial component of the Polycomb group (PcG) and serves as part of the PRC2/EED-EZH2 complex. This complex catalyzes methylation of histone H3 at lysine residues 9 and 27, leading to transcriptional repression of target genes including HOXC8, HOXA9, MYT1, and CDKN2A. EED also recognizes trimethylated histone H1 at lysine 26, which modulates PRC2 methyltransferase activity. Additionally, the PRC2 complex may facilitate recruitment of DNA methyltransferases, linking histone modification and DNA methylation epigenetic systems. EED is primarily localized in the nucleus and chromosomes, where it transiently colocalizes with XIST at inactive X chromosomes, underscoring its role in chromatin organization and gene silencing pathways.
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Protein name
Polycomb protein EED
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Uniprot ID
O75530
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Gene Name
EED
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 78-441 aa (O75530) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 43.64 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 44 kDa band.
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Purity
>86%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"