Human ENTPD5 (His Tag) recombinant protein

Human ENTPD5 (His Tag) recombinant protein

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Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

Ectonucleoside triphosphate diphosphohydrolase 5

GDPASE ENTPD5; NUCLEOSIDE DIPHOSPHATASE; PCPH; PROTO-ONCOGENE CPH; ER-UDPASE; URIDINE-DIPHOSPHATASE ENTPD5; CD39L4; AI987697; MNTPASE; CD39-LIKE 4; NTPDASE 5; NTPDASE-5; AI196558; CD39 ANTIGEN-LIKE 4; GUANOSINE-DIPHOSPHATASE ENTPD5; PCPH PROTO-ONCOGENE PROTEIN; NTPDASE5; UDPASE ENTPD5

O75356

ENTPD5; CD39L4; PCPH

Baculovirus-Insect Cells

A DNA sequence encoding the human ENTPD5 (O75356) (Met 1-His 428) was fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human ENTPD5 consists of 418 amino acids and predicts a molecular mass of 47 kDa. It migrates as an approximately 45 KDa band in SDS-PAGE in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its ability to hydrolyze the 5’phosphate groups from the substrate guanosine5’diphosphate (GDP).
The specific activity is >7,000 pmoles/min/μg.