E2F1 antibody

Cat.#: 110153

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Product Information

  • Product Name
    E2F1 antibody
  • Documents
  • Description
    E2F1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in mouse brain tissue, A431 cells, HeLa cells, NIH/3T3 cells. Observed molecular weight by Western-blot: 55 kDa
  • Tested applications
    ELISA, IHC, WB
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    E2F 1 antibody; E2F transcription factor 1 antibody; E2F1 antibody; PBR3 antibody; PRB binding protein E2F 1 antibody; RBAP 1 antibody; RBAP1 antibody; RBBP 3 antibody; RBBP3 antibody; Transcription factor E2F1 antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of E2F1 recombinant protein (Accession Number: NM_005225). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110153(E2F1 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:110153(E2F1 antibody) at dilution of 1:50 (under 10x lens)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110153(E2F1 Antibody) at dilution of 1:300

    mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110153(E2F1 Antibody) at dilution of 1:300

  • Background
    Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of E2F1 protein, specifically recognizes the 47kd human E2F1 protein.
  • References
    • Xu F, You X, Liu F. The oncoprotein HBXIP up-regulates Skp2 via activating transcription factor E2F1 to promote proliferation of breast cancer cells. Cancer letters. 333(1):124-32. 2013.
    • Valle BL, D'Souza T, Becker KG. Non-steroidal anti-inflammatory drugs decrease E2F1 expression and inhibit cell growth in ovarian cancer cells. PloS one. 8(4):e61836. 2013.
    • Zhang T, Zhang J, Cui M. Hepatitis B virus X protein inhibits tumor suppressor miR-205 through inducing hypermethylation of miR-205 promoter to enhance carcinogenesis. Neoplasia (New York, N.Y.). 15(11):1282-91. 2013.
    • Sun Q, Wang R, Wang Y, Luo J, Wang P, Cheng B. Notch1 is a potential therapeutic target for the treatment of human hepatitis B virus X protein-associated hepatocellular carcinoma. Oncology reports. 31(2):933-9. 2014.
    • Hu Z, Ding W, Zhu D. TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy. The Journal of clinical investigation. 125(1):425-36. 2015.
    • Liu M, Chen Y, Zhang L. Regulation of Hepatic Cholesteryl Ester Transfer Protein Expression and Reverse Cholesterol Transport by Inhibition of DNA Topoisomerase II. The Journal of biological chemistry. 290(23):14418-29. 2015.
    • Liu J, Wang M, Zhang X. CIP2A is associated with multidrug resistance in cervical adenocarcinoma by a P-glycoprotein pathway. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"