E.coli Tryptophan synthetase (His tag) recombinant protein

E.coli Tryptophan synthetase (His tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P). Indole is then transferred through a 25-Å hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

Tryptophan synthase alpha chain & Tryptophan synthase beta chain

P0A877&P0A879

E.coli

A DNA sequence encoding the E.coli Tryptophan synthetase Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus.

E.coli

>95% as determined by SDS-PAGE.