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Product Name
DYNC1I2 antibody
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Description
DYNC1I2 Rabbit Polyclonal antibody. Positive WB detected in human brain tissue, A375 cells, mouse testis tissue. Positive IP detected in mouse testis tissue. Positive IF detected in A375 cells. Observed molecular weight by Western-blot: 68 kDa and 72 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DH IC 2 antibody; DNCI2 antibody; DNCIC2 antibody; DYNC1I2 antibody; FLJ21089 antibody; FLJ90842 antibody; IC2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of DYNC1I2 recombinant protein (Accession Number: NM_001271788). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:110136(DYNC1I2 antibody) at dilution of 1:500
IP Result of anti-DYNC1I2 (IP:Catalog No:110136, 4ug; Detection:Catalog No:110136 1:500) with mouse testis tissue lysate 4000ug.
Immunofluorescent analysis of A375 cells using Catalog No:110136(DYNC1I2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
DYNC1I2 represents dynein, cytoplasmic 1, intermediate chain 2
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References
- Inaba H, Goto H, Kasahara K. Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway. The Journal of cell biology. 212(4):409-23. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"