DOT1L Polyclonal Antibody

Cat.#: 165458

Special Price 174.2 USD

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Product Information

  • Product Name
    DOT1L Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to DOT1L
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    DOT1L antibody; DOT1 antibody; KMT4 antibody; DOT1 like histone lysine methyltransferase antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-500 of human DOT1L (NP_115871.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - DOT1L Polyclonal Antibody

    Western blot - DOT1L Polyclonal Antibody

    Western blot analysis of extracts of HeLa cells, using DOT1L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

  • Background
    Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"