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Product Name
DNTTIP1 Polyclonal Antibody
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Description
Polyclonal antibody to DNTTIP1
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
DNTTIP1 antibody; C20orf167 antibody; Tdif1 antibody; dJ447F3.4 antibody; deoxynucleotidyltransferase terminal-interacting protein 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-329 of human DNTTIP1 (NP_443183.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:200 - 1:2000
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Validations
Western blot - DNTTIP1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using DNTTIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5S.
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Background
Increases DNTT terminal deoxynucleotidyltransferase activity (in vitro). Also acts as a transcriptional regulator, binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription. Binds DNA and nucleosomes; may recruit HDAC1 complexes to nucleosomes or naked DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"