DNMT3B Polyclonal Antibody

Cat.#: 165267

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Product Information

  • Product Name
    DNMT3B Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to DNMT3B
  • Tested applications
    WB, IHC, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    DNMT3B antibody; ICF antibody; ICF1 antibody; M.HsaIIIB antibody; DNA methyltransferase 3 beta antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant protein of human DNMT3B
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IHC 1:50 - 1:200
    IF 1:50 - 1:200
  • Validations

    Western blot - DNMT3B Polyclonal Antibody

    Western blot - DNMT3B Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using DNMT3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunohistochemistry - DNMT3B Polyclonal Antibody

    Immunohistochemistry - DNMT3B Polyclonal Antibody

    Immunohistochemistry of paraffin-embedded rat testis using DNMT3B antibody at dilution of 1:100 (40x lens).

  • Background
    Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"