DKK1 antibody

Cat.#: 109982

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Product Information

  • Product Name
    DKK1 antibody
  • Documents
  • Description
    DKK1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Positive WB detected in human placenta tissue, HeLa cells, K-562 cells, Sp2/0 cells. Observed molecular weight by Western-blot: 30-35 kDa
  • Tested applications
    ELISA, WB, IF, IHC
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    Dickkopf 1 antibody; Dickkopf related protein 1 antibody; DKK 1 antibody; DKK1 antibody; hDkk 1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DKK1 recombinant protein (Accession Number: NM_012242). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:109982(DKK1 antibody) at dilution of 1:500

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:109982(DKK1 antibody) at dilution of 1:500

    Immunofluorescent analysis of HepG2 cells, using DKK1 antibody Catalog No:109982 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunofluorescent analysis of HepG2 cells, using DKK1 antibody Catalog No:109982 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

    Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:109982(DKK1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:109982(DKK1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:109982(DKK1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:109982(DKK1 Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kD doublet protein, which is larger than the deduced molecular mass of 26 kD. Sometime DKK1 is expressed as a 42- to 50-kD secreted protein, with little change observed after glycanase treatment.
  • References
    • Ying Q, Ansong E, Diamond AM, Lu Z, Yang W, Bie X. Quantitative proteomic analysis reveals that anti-cancer effects of selenium-binding protein 1 in vivo are associated with metabolic pathways. PloS one. 10(5):e0126285. 2015.
    • Liu BL, Sun KX, Zong ZH, Chen S, Zhao Y. MicroRNA-372 inhibits endometrial carcinoma development by targeting the expression of the Ras homolog gene family member C (RhoC). Oncotarget. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"