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Product Name
Recombinant DIESL protein (His tag)
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Description
Recombinant DIESL/TMEM68 (147-324) is a high-purity, soluble protein fragment derived from the human transmembrane protein 68. This fragment, representing a stable and structurally defined region of the enzyme, is ideal for investigating the alternative triglyceride biosynthesis pathway and its role in lipid metabolism.
The full-length DIESL/TMEM68 protein functions as a catalytic subunit in an alternative triglyceride biosynthesis pathway, synthesizing triacylglycerol from diacylglycerol and membrane phospholipids such as phosphatidylcholine. This activity alters membrane composition and supports mitochondrial function during lipid starvation, providing energy for rapid growth under fuel-limited conditions. Additionally, it exhibits acyl-CoA:monoacylglycerol acyltransferase (MGAT) and acyl-CoA:diacylglycerol acyltransferase (DGAT) activities. The protein is localized to the endoplasmic reticulum membrane as a multi-pass transmembrane protein, underscoring its integral role in cellular lipid homeostasis.
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Protein name
DGAT1/2-independent enzyme synthesizing storage lipids
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Uniprot ID
Q96MH6
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Gene Name
TMEM68
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 147-324 aa (Q96MH6) were fused with 6His tag.
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Sequence
147-324
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 21.92 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 22 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"