Dermaseptin-B4 peptide

Dermaseptin-B4 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-ALWKDILKNVGKAAGKAVLNTVTDMVNQ-NH2

H-Ala-Leu-Trp-Lys-Asp-Ile-Leu-Lys-Asn-Val-Gly-Lys-Ala-Ala-Gly-Lys-Ala-Val-Leu-Asn-Thr-Val-Thr-Asp-Met-Val-Asn-Gln-NH2

28

95.06%

C₁₃₃H₂₂₆N₃₈O₃₈S

2997.5

Synthetic

Powder

The B4 peptide, or dermaseptin B4, is one of the peptides belonging to the dermaseptin B family isolated from the skin secretions of the South American frog Phyllomedusa bicolor. Synthetic dermaseptin B4 is highly active in inhibiting the growth of Gram - positive and Gram - negative bacteria, with minimal inhibitory concentrations in the range of 1 - 10μM. It shows cytotoxicity against mammalian cells at doses above 10μM, with 50% inhibition at 20μM.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Charpentier S, Amiche M, Mester J, Vouille V, Le Caer JP, Nicolas P, Delfour A. Structure, synthesis, and molecular cloning of dermaseptins B, a family of skin peptide antibiotics. J Biol Chem. 1998 Jun 12;273(24):14690-7. doi: 10.1074/jbc.273.24.14690. PMID: 9614066.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.