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DDRGK1 antibody

Cat.#: 109777

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Product Information

  • Product Name
    DDRGK1 antibody
  • Documents
  • Description
    DDRGK1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in mouse brain tissue, human pancreas tissue. Positive WB detected in mouse liver tissue, mouse brain tissue, mouse spleen tissue. Observed molecular weight by Western-blot: 36-46 kDa
  • Tested applications
    ELISA, WB, IHC, IF
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    C20orf116 antibody; DDRGK domain containing 1 antibody; DDRGK1 antibody; dJ1187M17.3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of DDRGK1 recombinant protein (Accession Number: NM_023935). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109777(DDRGK1 Antibody) at dilution of 1:600

    mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109777(DDRGK1 Antibody) at dilution of 1:600

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:109777(DDRGK1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:109777(DDRGK1 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:109777(DDRGK1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded mouse brain tissue slide using Catalog No:109777(DDRGK1 Antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells using Catalog No:109777(DDRGK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:109777(DDRGK1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    DDRGK1, also name as C20orf116, is a member of the DDRGK domain-containing protein family with unknown function. Depletion of DDRGK1 inhibits cell proliferation and invasion. Expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. DDRGK1 acts as an important regulator of the NF-κB pathway. (PMID: 23675531) Catalog#21445-1-AP is a rabbit polyclonal antibody raised against the full-length of human DDRGK1.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"