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  • DDHD2 antibody
  • DDHD2 antibody

DDHD2 antibody

Cat.#: 109772

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Special Price 239.0 USD

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Product Information

  • Product Name
    DDHD2 antibody
  • Documents
  • Description
    DDHD2 Rabbit Polyclonal antibody. Positive IF detected in HEK-293 cells. Positive WB detected in rat brain tissue, mouse lung tissue, mouse testis tissue. Observed molecular weight by Western-blot: 80-90 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    DDHD domain containing 2 antibody; DDHD2 antibody; KIAA0725 antibody; KIAA0725p antibody; Phospholipase DDHD2 antibody; SAMWD1 antibody
  • Immunogen
  • Isotype
    IgG
  • Preparation
    This antibody was obtained by immunization of DDHD2 recombinant protein (Accession Number: BC010504). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:1000-1:10000

    IF: 1:10-1:100

  • Validations

    rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109772(DDHD2 Antibody) at dilution of 1:2000

    rat brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109772(DDHD2 Antibody) at dilution of 1:2000

    Immunofluorescent analysis of HEK-293 cells using Catalog No:109772(DDHD2 Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HEK-293 cells using Catalog No:109772(DDHD2 Antibody) at dilution of 1:25 and Alexa Fluor 594-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    DDHD2, also known as KIAA0725p, is a member of the intracellular phospholipase A1 (PLA1) protein family which comprises a group of enzymes that hydrolyze the sn-1 ester bond of phospholipids, producing 2-acyl-lysophospholipids and fatty acids. DDHD2 prefers phosphatidic acid as substrate and has a role in efficient membrane trafficking from the Golgi apparatus to the plasma membrane. The gene of DDHD2 maps to chromosome 8p11.23, and encodes a 711-amino-acid protein with a calculated molecular mass of 81 kDa. It has been reported that DDHD2 immunoblot analysis of various tissue extracts revealed two bands of 90 and 85 kDa (PMID: 11788596).
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"