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Product Name
Recombinant DAPP1 protein (His tag)
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Description
Recombinant Human DAPP1 (hDAPP1) fragment (amino acids 1-280), a dual adapter protein that plays a key role in B-cell antigen receptor signaling downstream of PI3K. This fragment encompasses the full-length SH2 and PH domains, making it an ideal tool for investigating domain-specific functions in immune signaling pathways.
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide (hDAPP1), also known as B lymphocyte adapter protein Bam32, functions as a B-cell-associated adapter regulating B-cell antigen receptor (BCR)-signaling downstream of PI3K. The protein localizes to the cytoplasm and membrane, where it becomes membrane-associated following cell stimulation, facilitating its translocation. This membrane recruitment mechanism underscores its importance in coordinating signaling events in B-cell immune responses.
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Protein name
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
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Uniprot ID
Q9UN19
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Gene Name
DAPP1; BAM32
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-280 aa (Q9UN19) were fused with N-terminal 6His tag.
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Sequence
MSKIKHHHHH HGGSMGRAEL LEGKMSTQDP SDLWSRSDGE AELLQDLGWY HGNLTRHAAE ALLLSNGCDG SYLLRDSNET TGLYSLSVRA KDSVKHFHVE YTGYSFKFGF NEFSSLKDFV KHFANQPLIG SETGTLMVLK HPYPRKVEEP SIYESVRVHT AMQTGRTEDD LVPTAPSLGT KEGYLTKQGG LVKTWKTRWF TLHRNELKYF KDQMSPEPIR ILDLTECSAV QFDYSQERVN CFCLVFPFRT FYLCAKTGVE ADEWIKILRW KLSQIRKQLN QGEGTIRSRS FIFK
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 33.79 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 36 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Validations
Recombinant DAPP1 protein (His tag) SDS-PAGE
M: Marker; Lane 1: DAPP1; Lane 2: BSA
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