D2HGDH Rabbit Polyclonal antibody. Positive IHC detected in human liver cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse liver tissue. Positive WB detected in human liver tissue, human heart tissue, mouse kidney tissue, mouse liver tissue, rat liver tissue. Observed molecular weight by Western-blot: 56 kDa
ELISA, WB, IHC, IP, IF
Human,Mouse,Rat; other species not tested.
D2HGD antibody; D2HGDH antibody; FLJ42195 antibody
This antibody was obtained by immunization of D2HGDH recombinant protein (Accession Number: NM_152783). Purification method: Antigen affinity purified.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109817(D2HGDH antibody) at dilution of 1:500
IP result of anti-D2HGDH (Catalog No:109817 for IP and Detection) with mouse liver tissue lysate.
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109817(D2HGDH antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109817(D2HGDH antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-D2HGDH (IP:Catalog No:109817, 3ug; Detection:Catalog No:109817 1:500) with mouse liver tissue lysate 5000ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:109817(D2HGDH Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.
- Intlekofer AM, Dematteo RG, Venneti S. Hypoxia Induces Production of L-2-Hydroxyglutarate. Cell metabolism. 22(2):304-11. 2015.
- Krell D, Assoku M, Galloway M, Mulholland P, Tomlinson I, Bardella C. Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma. PloS one. 6(5):e19868. 2011.
- Ma S, Jiang B, Deng W. D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth. Oncotarget. 6(11):8606-20. 2015.
- Lin AP, Abbas S, Kim SW. D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2. Nature communications. 6:7768. 2015.
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