D-LAK-120A (D-LAK) peptide

D-LAK-120A (D-LAK) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KKLALALAKKWLALAKKLALALAKK-NH2 (All D-type amino acids)

H-Lys-Lys-Leu-Ala-Leu-Ala-Leu-Ala-Lys-Lys-Trp-Leu-Ala-Leu-Ala-Lys-Lys-Leu-Ala-Leu-Ala-Leu-Ala-Lys-Lys-NH2 (All D-type amino acids)

25

95.18%

C₁₃₁H₂₃₇N₃₅O₂₅

2702.49

Synthetic

Powder

D-LAK-120A (D-LAK; KKLALALAKKWLALAKKLALALAKK-NH2) a cationic antimicrobial peptide. The D-LAK peptides were cationic and amphipathic, with angle subtended by positively charged lysine residues when the peptide adopts an idealized α-helix conformation. D-LAK showed inhibitory action against M. smegmatis by acting on the mycobacterial cell wall and leading to pore formation (membranolytic pathway) in vitro. D-LAK peptide demonstrated enhanced cytotoxicity in A549, H358, H1975, and HCC827 cell lines with inhibitory concentrations between 4.0 and 5.5 μM. D-LAK induced lung cancer cell apoptosis and arrested cells in the S phase (DNA synthesis) of cell cycle. D-LAK inhibited single cell proliferation and cancer cell migration in vitro. The tumor reduction observed in the 3D spheroid assay further suggests the potential use of D-LAK as an anticancer agent for NSCLC treatment.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Suyash M. Patil, Nitesh K. Kunda, Anticancer activity of D-LAK-120A, an antimicrobial peptide, in non-small cell lung cancer (NSCLC), Biochimie, 2022, ISSN 0300-9084, https://doi.org/10.1016/j.biochi.2022.06.011.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.