D-LAK-120A (D-LAK) peptide

D-LAK-120A (D-LAK) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KKLALALAKKWLALAKKLALALAKK-NH2 (All D-type amino acids)

H-Lys-Lys-Leu-Ala-Leu-Ala-Leu-Ala-Lys-Lys-Trp-Leu-Ala-Leu-Ala-Lys-Lys-Leu-Ala-Leu-Ala-Leu-Ala-Lys-Lys-NH2 (All D-type amino acids)

25

95.18%

C₁₃₁H₂₃₇N₃₅O₂₅

2702.49

Synthetic

Powder

D-LAK-120A (D-LAK; KKLALALAKKWLALAKKLALALAKK-NH2) a cationic antimicrobial peptide. The D-LAK peptides were cationic and amphipathic, with angle subtended by positively charged lysine residues when the peptide adopts an idealized α-helix conformation. D-LAK showed inhibitory action against M. smegmatis by acting on the mycobacterial cell wall and leading to pore formation (membranolytic pathway) in vitro. D-LAK peptide demonstrated enhanced cytotoxicity in A549, H358, H1975, and HCC827 cell lines with inhibitory concentrations between 4.0 and 5.5 μM. D-LAK induced lung cancer cell apoptosis and arrested cells in the S phase (DNA synthesis) of cell cycle. D-LAK inhibited single cell proliferation and cancer cell migration in vitro. The tumor reduction observed in the 3D spheroid assay further suggests the potential use of D-LAK as an anticancer agent for NSCLC treatment.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Suyash M. Patil, Nitesh K. Kunda, Anticancer activity of D-LAK-120A, an antimicrobial peptide, in non-small cell lung cancer (NSCLC), Biochimie, 2022, ISSN 0300-9084, https://doi.org/10.1016/j.biochi.2022.06.011.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.