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  • Cystatin B antibody
  • Cystatin B antibody
  • Cystatin B antibody
  • Cystatin B antibody
  • Cystatin B antibody

Cystatin B antibody

Cat.#: 107657

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Special Price 239.0 USD

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Product Information

  • Product Name
    Cystatin B antibody
  • Documents
  • Description
    Cystatin B Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive IP detected in MCF-7 cells. Positive WB detected in human placenta tissue, HepG2 cells, human liver tissue, human lymph tissue, human spleen tissue, MCF7 cells. Observed molecular weight by Western-blot: 11 kDa
  • Tested applications
    ELISA, WB, IHC, IF, FC, IP
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CPI B antibody; CST6 antibody; CSTB antibody; Cystatin B antibody; cystatin B (stefin B) antibody; EPM1 antibody; PME antibody; Stefin B antibody; STFB antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of Cystatin B recombinant protein (Accession Number: BC003370). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107657(CSTB antibody) at dilution of 1:1500

    human placenta tissue were subjected to SDS PAGE followed by western blot with Catalog No:107657(CSTB antibody) at dilution of 1:1500

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:107657(CSTB antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:107657(CSTB antibody) at dilution of 1:100 (under 10x lens)

    Immunofluorescent analysis of HepG2 cells, using CSTB antibody Catalog No:107657 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using CSTB antibody Catalog No:107657 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-CSTB (IP:Catalog No:107657, 4ug; Detection:Catalog No:107657 1:4000) with MCF-7 cells lysate 1320ug.

    IP Result of anti-CSTB (IP:Catalog No:107657, 4ug; Detection:Catalog No:107657 1:4000) with MCF-7 cells lysate 1320ug.

    1X10^6 HepG2 cells were stained with 0.2ug CSTB antibody (Catalog No:107657, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with 0.2ug CSTB antibody (Catalog No:107657, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    CSTB represents cystatin B (stefin B)
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"