CT20p peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

The CT20 peptide, derived from the C terminus of Bax, is a hydrophobic peptide that shares similarities with antimicrobial peptides. It localizes to mitochondria in cancer cells, leading to fusion-like aggregation, mitochondrial membrane hyperpolarization, and impaired mitochondrial movement. This disruption in mitochondrial dynamics results in decreased ATP levels and altered cytoskeletal organization, including reduced F-actin polymerization. Notably, CT20p exhibits selective activity towards cancer cells, as it does not associate with mitochondria in normal cells, leaving their mitochondrial membrane potential, morphology, and localization unaffected.

CT20p's mechanism involves the reduction of integrin expression, particularly the a5b1 complex, which precedes cell detachment. The peptide's ability to specifically target cancer cell mitochondria and disrupt their function highlights its potential as a tool for studying mitochondrial dynamics in malignant cells. Its hydrophobic nature necessitates delivery via nanoparticles for cellular uptake, enabling its intracellular actions while maintaining selectivity for cancerous over non-cancerous cells.

• Lee, M., Bassiouni, R., Sparrow, N. et al. The CT20 peptide causes detachment and death of metastatic breast cancer cells by promoting mitochondrial aggregation and cytoskeletal disruption. Cell Death Dis 5, e1249 (2014). https://doi.org/10.1038/cddis.2014.225

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.