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Product Name
Recombinant CSP1 protein (His tag)
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Description
This recombinant protein product features a stable and soluble fragment (residues 283-408) of Caspase A (EC 3.4.22.36) from Caenorhabditis elegans, encoded by the csp-1 gene. The fragment is ideal for structural studies, biochemical assays, and functional investigations of caspase activity and apoptosis pathways.
Caspase A is a cysteine protease that cleaves itself and caspase ced-3 into their mature active forms in vitro, and it also processes inactive caspase csp-2 isoform b. It plays a critical role in inducing apoptosis during embryogenesis in C. elegans, largely independent of the canonical ced-9, ced-4, and ced-3 apoptosis pathway. Additionally, Caspase A is involved in the degeneration of dopaminergic CEP neurons under high Mn²⁺ conditions. Isoform a of the protein is dispensable for embryogenic apoptosis regulation. The provided fragment was selected computationally for its stability and solubility, facilitating research into caspase mechanisms and neuronal degeneration.
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Protein name
Caspase A
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Uniprot ID
G5EBM1
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Gene Name
csp-1
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 283-408 aa (G5EBM1) were fused with 6His tag.
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Sequence
283-408
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Protein Species
Caenorhabditis elegans
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Molecular weight
Predictes a molecular mass of 15.95 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 16 kDa band.
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Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"