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Product Name
CRIPT antibody
- Documents
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Description
CRIPT Rabbit Polyclonal antibody. Positive IF detected in L02 cells. Positive WB detected in L02 cells. Positive IP detected in L02 cells. Observed molecular weight by Western-blot: 15 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CRIPT antibody; HSPC139 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CRIPT recombinant protein (Accession Number: NM_014171). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:109562(CRIPT antibody) at dilution of 1:500
IP Result of anti-CRIPT (IP:Catalog No:109562, 4ug; Detection:Catalog No:109562 1:500) with L02 cells lysate 1800ug.
Immunofluorescent analysis of L02 cells using Catalog No:109562(CRIPT Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The clustering and immobilization of receptors and ion channels located at specific subcellular sites are believed to be mediated by intracellular proteins that attach these membrane proteins to the cytoskeleton. Receptors and ion channels interact with cytoplasmic proteins, which involved in the modulation or downstream signaling mechanisms of the receptor/ion channel. CRIPT is the protein that binds to the third PDZ (PDZ3) domain of PSD95, which binds to and clusters a variety of membrane proteins, including Shaker-type potassium channel subunits and NMDA receptor NR2 subunits, via its first 2 N-terminal PDZ domains
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"