-
Product Name
CREM Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to CREM
-
Tested applications
WB, IHC
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
CREM antibody; CREM-2 antibody; ICER antibody; hCREM-2 antibody; cAMP-responsive element modulator antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CREM (NP_853549.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CREM Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CREM antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.
Immunohistochemistry - CREM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human mammary cancer using CREM antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - CREM Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human placenta using CREM antibody at dilution of 1:100 (40x lens).
-
Background
Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation.; Isoform 6: May play a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"