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Product Name
CPT1B Polyclonal Antibody
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Description
Polyclonal antibody to CPT1B
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Tested applications
WB, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
CPT1B antibody; CPT1-M antibody; CPT1M antibody; CPTI antibody; CPTI-M antibody; M-CPT1 antibody; MCCPT1 antibody; MCPT1 antibody; carnitine palmitoyltransferase 1B antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IF 1:50 - 1:200 -
Validations
Western blot - CPT1B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CPT1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Immunofluorescence - CPT1B Polyclonal Antibody
Immunofluorescence analysis of NIH/3T3 cells using CPT1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - CPT1B Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using CPT1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"