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Product Name
COQ7 antibody
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Description
COQ7 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in mouse liver tissue, mouse heart tissue. Positive IHC detected in human liver cancer tissue. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAT5 antibody; CLK 1 antibody; CLK1 antibody; COQ7 antibody; Timing protein clk 1 homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of COQ7 recombinant protein (Accession Number: XM_047433494). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:109472(COQ7 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109472(COQ7 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:109472(COQ7 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-COQ7 (IP:Catalog No:109472, 4ug; Detection:Catalog No:109472 1:500) with mouse liver tissue lysate 6000ug.
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Background
COQ7(Ubiquinone biosynthesis protein COQ7 homolog) catalyzes the production of coenzyme Q (CoQ) in mitochondria. The predicted protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and 3 N-myristoylation sites. This protein has 2 isoforms produced by alternative splicing.
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References
- Ashraf S, Gee HY, Woerner S. ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption. The Journal of clinical investigation. 123(12):5179-89. 2013.
- Monaghan RM, Barnes RG, Fisher K. A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity. Nature cell biology. 17(6):782-92. 2015.
- Danhauser K, Herebian D, Haack TB. Fatal neonatal encephalopathy and lactic acidosis caused by a homozygous loss-of-function variant in COQ9. European journal of human genetics : EJHG. 24(3):450-4. 2016.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"