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Product Name
Human Complement Component C2 (His Tag) recombinant protein
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Description
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor C4b to generate the C3 or C5 convertase.
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Protein name
Complement C2
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Protein short names
DKFZP779M0311; CO2; DADB-122G4.1; C2; RP24-317H19.3; ARMD14
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Uniprot ID
Q5JP69
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Gene Name
C2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human complement component 2 (C2) precursor (NP_000054.2) (Met 1-Leu 752) was expressed with a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The single-chain form of recombinant human complement component C2 comprises 743 amino acids and has a calculated molecular mass of 82.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhC2 is 90-100 kDa as a result of glycosylation.
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Purity
> 97 % as determined by SDS-PAGE and SEC-HPLC Analysis.
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Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithiobis (2-nitrobenzoic acid) (DTNB). The specific activity is >100 pmoles/min/μg.
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Validations
Human C2 / Complement Component 2 Protein (His Tag) SDS-PAGE
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