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Product Name
Collagen Type III antibody
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Description
Collagen Type III Rabbit Polyclonal antibody. Positive IP detected in mouse skin tissue. Positive WB detected in mouse skin tissue. Positive IHC detected in human colon tissue, human skin tissue. Observed molecular weight by Western-blot: 140-150 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
COL3A1 antibody; Collagen alpha 1(III) chain antibody; Collagen Type III antibody; collagen antibody; type III antibody; alpha 1 antibody; EDS4A antibody; FLJ34534 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Collagen Type III recombinant protein (Accession Number: BC028178). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse skin tissue were subjected to SDS PAGE followed by western blot with Catalog No:109377(COL3A1 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:109377(COL3A1 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:109377(COL3A1 Antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-COL3A1 (IP:Catalog No:109377, 4ug; Detection:Catalog No:109377 1:500) with mouse skin tissue lysate 3200ug.
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Background
Type III collagen is a fibrillar forming collagen comprising three a1(III) chains and is expressed in early embryos and throughout embryogenesis. In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen. COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with type III and IV Ehlers-Danlos syndrome, and with aortic and arterial aneurysms. (PMID: 9050868; 2445760)
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