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Product Name
Cofilin antibody
- Documents
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Description
Cofilin Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, human brain tissue, human skin tissue, MCF7 cells, NIH/3T3 cells, rat brain tissue. Positive IHC detected in human cervical cancer tissue, human heart tissue, human lung cancer tissue. Positive IF detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
18 kDa phosphoprotein antibody; CFL antibody; CFL1 antibody; Cofilin 1 antibody; cofilin 1 (non muscle) antibody; Cofilin antibody; non muscle isoform antibody; p18 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Cofilin recombinant protein (Accession Number: NM_005507). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:109365(CFL1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109365(CFL1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human cervical cancer using Catalog No:109365(CFL1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed NIH/3T3 cells using Catalog No:109365(Cofilin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation.Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
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References
- Zhang C, Zhang W, Lu Y. NudC regulates actin dynamics and ciliogenesis by stabilizing cofilin 1. Cell research. 26(2):239-53. 2016.
- Yang X, Zou P, Yao J. Proteomic dissection of cell type-specific H2AX-interacting protein complex associated with hepatocellular carcinoma. Journal of proteome research. 9(3):1402-15. 2010.
- Wang WS, Zhong HJ, Xiao DW. The expression of CFL1 and N-WASP in esophageal squamous cell carcinoma and its correlation with clinicopathological features. Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E. 23(6):512-21. 2010.
- Cao HH, Zhang SY, Shen JH. A three-protein signature and clinical outcome in esophageal squamous cell carcinoma. Oncotarget. 6(7):5435-48. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"