CM-10 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

CM-10, with the amino acid sequence Tyr-Pro-Phe-Pro-Gly-Pro-Ile-Pro-Asn-Ser (YPFPGPIPNS), is a peptide identified as β-casomorphin-10, derived from residues 60–69 of bovine β-casein. It is released from casein through enzymatic hydrolysis using the food-grade protease Aspergillus oryzae protease ASD. This peptide belongs to the class of exogenous opioid peptides and exhibits agonistic activity for both δ-opioid (DOR) and μ-opioid (MOR) receptors, as demonstrated in receptor-expressing cell assays. Structural analyses, including molecular docking simulations, indicate that its compact conformation, influenced by multiple proline residues, facilitates interaction with the opioid receptor binding pockets.

In vitro characterization shows that CM-10 and related β-casomorphin derivatives interact with opioid receptors. Studies involving intravital calcium imaging in transgenic mouse models have demonstrated that CM-10 induces transient Ca²⁺ signaling in the epithelial cells of the small intestinal villi. This signaling response is inhibited by co-administration of selective δ- or μ-opioid receptor antagonists, indicating a specific interaction with gut-located opioid receptors. The peptide's structure contributes to its stability against proteolytic degradation.

• Takeuchi Y, Fukunaga M, Iwatani S, Miyanaga K, Adachi T, Yamamoto N. Release of an anti-anxiety peptide in casein hydrolysate with Aspergillus oryzae protease. Food Funct. 2022;13(20):10449-10460

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.