Recombinant CLOCK protein (His tag)

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Recombinant Human CLOCK Protein Fragment (1-391) is a high-purity, functionally active protein encompassing the essential bHLH, PAS 1, PAS 2, and PAC domains. This fragment serves as a critical tool for investigating the core transcriptional mechanisms of the circadian clock, enabling researchers to study domain-specific interactions, DNA binding, and heterodimerization with partners like BMAL1 in circadian regulation and related physiological processes.

The full-length Circadian locomoter output cycles protein kaput (CLOCK) is a central transcriptional activator in the mammalian circadian clock, which governs 24-hour rhythms in gene expression to regulate metabolism, sleep, immune function, and cardiovascular health. It forms heterodimers with BMAL1 or BMAL2 to activate transcription of core clock genes and clock-controlled genes via E-box motifs, while also possessing intrinsic acetyltransferase activity that remodels chromatin and modifies metabolic enzymes. CLOCK shuttles between the nucleus and cytoplasm in a circadian-dependent manner, with its phosphorylated form localized primarily in the nucleus. Disruptions in CLOCK function are linked to diseases such as cancer, metabolic syndromes, and aging, highlighting its broad physiological significance.