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Product Name
Recombinant CLK2 protein (His tag)
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Description
Recombinant Human CLK2 Kinase Domain (aa 142-499) is a high-purity protein fragment encompassing the complete protein kinase domain of Dual specificity protein kinase CLK2. This fragment serves as an essential tool for investigating kinase activity, substrate interactions, and regulatory mechanisms in splicing regulation and metabolic pathways.
Dual specificity protein kinase CLK2 (CDC-like kinase 2) is a human nuclear kinase that phosphorylates both serine/threonine and tyrosine residues on substrates, notably serine- and arginine-rich (SR) spliceosomal proteins. It regulates RNA splicing by redistributing SR proteins between nuclear speckles and nucleoplasm and suppresses hepatic gluconeogenesis by repressing PPARGC1A activity. CLK2 also phosphorylates PPP2R5B to promote PP2A-mediated AKT1 dephosphorylation and modulates alternative splicing of tissue factor in endothelial cells. The protein localizes to the nucleus, with specific isoforms accumulating in nuclear speckles, where it co-localizes with SR proteins to orchestrate splicing machinery.
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Protein name
Dual specificity protein kinase CLK2
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Uniprot ID
P49760
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Gene Name
CLK2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 142-499 aa (P49760) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 43.87 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 47 kDa band.
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Purity
>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"