CLE45 peptide

CLE45 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RRVRRGSDPIHN-OH

H-Arg-Arg-Val-Arg-Arg-Gly-Ser-Asp-Pro-Ile-His-Asn-OH

12

95.66%

C₅₉H₁₀₃N₂₇O₁₇

1462.61

Synthetic

Powder

CLE45 is a peptide related to CLAVATA3/EMBRYO SURROUNDING REIGON. It is genuinely expressed in developing protophloem sieve elements (PPSEs). Autocrine CLE45 signaling through BAM3 must be dampened below a threshold level to permit protophloem differentiation. It also acts paracrine to keep neighboring cell files in a pluripotent state through signaling via RPK2. The wild type becomes insensitive to CLE45 treatment on neutral to alkaline pH media, and pH-dependent perception specifically requires the CLE45 R4 residue and is not observed for CLE25 and CLE26. CLE45 is a potent inhibitor of protophloem formation and root growth. It has a different structure from CLE25 and CLE26, differing from CLE26 in positions 4 and 7, and from CLE25 in multiple positions. A synthetic mutant variant with certain residue replacements shows different behaviors related to pH dependence and receptor recognition.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Diaz-Ardila HN, Gujas B, Wang Q, Moret B, Hardtke CS. pH-dependent CLE peptide perception permits phloem differentiation in Arabidopsis roots. Curr Biol. 2023 Feb 6;33(3):597-605.e3. doi: 10.1016/j.cub.2022.12.056. Epub 2023 Jan 23. PMID: 36693368.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.