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Product Name
CLCN2 Polyclonal Antibody
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Description
Polyclonal antibody to CLCN2
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
CLCN2 antibody; CIC-2 antibody; CLC2 antibody; ECA2 antibody; ECA3 antibody; EGI11 antibody; EGI3 antibody; EGMA antibody; EJM6 antibody; EJM8 antibody; LKPAT antibody; clC-2 antibody; chloride channel protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human CLCN2 (NP_004357.3).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - CLCN2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CLCN2 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Involved in the regulation of aldosterone production. The opening of CLCN2 channels at hyperpolarized membrane potentials in the glomerulosa causes cell membrane depolarization, activation of voltage-gated Ca2+ channels and increased expression of aldosterone synthase, the rate-limiting enzyme for aldosterone biosynthesis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"