Product Name

Citrate synthase antibody

Documents

Description

Citrate synthase Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue. Positive WB detected in HEK-293 cells, HeLa cells, mouse skeletal muscle tissue. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 50 kDa

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

Citrate synthase antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Citrate synthase recombinant protein (Accession Number: NM_004077). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:1000-1:10000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:109325(CS antibody) at dilution of 1:2000

Immunohistochemical of paraffin-embedded human heart using Catalog No:109325(CS antibody) at dilution of 1:100 (under 10x lens)

Immunohistochemical of paraffin-embedded human heart using Catalog No:109325(CS antibody) at dilution of 1:100 (under 40x lens)

Immunofluorescent analysis of HepG2 cells, using CS antibody Catalog No:109325 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-CS (IP:Catalog No:109325, 4ug; Detection:Catalog No:109325 1:1000) with mouse heart tissue lysate 3200ug.

Background

Citrate synthase (CS), the first and rate-limiting enzyme of the tricarboxylic acid cycle, plays a key role in regulating energy generation of mitochondrial respiration(PMID:19479947).It belongs to the citrate synthase family. The deduced 466-amino acid protein contains an N-terminal mitochondrial targeting sequence and a motif highly conserved in citrate synthases(PMID:12549038). It can exsit as a dimer(PMID:8749851). Northern blot analysis detected no CS expression in thymus and small intestine(PMID:12549038). This antibody is specific to CS.

References

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Rhein VF, Carroll J, He J, Ding S, Fearnley IM, Walker JE. Human METTL20 methylates lysine residues adjacent to the recognition loop of the electron transfer flavoprotein in mitochondria. The Journal of biological chemistry. 289(35):24640-51. 2014.
Ma X, Li C, Sun L. Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Nature communications. 5:5212. 2014.
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Fong MY, Zhou W, Liu L. Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis. Nature cell biology. 17(2):183-94. 2015.
Oliva CR, Markert T, Gillespie GY, Griguer CE. Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas. Oncotarget. 6(6):4330-44. 2015.

Citrate synthase antibody

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